Journal: Journal of Cellular and Molecular Medicine

Article Title: Investigating Liquid–Liquid Phase Separation in Lung Adenocarcinoma to Improve Prognostic Accuracy and Treatment Efficacy

doi: 10.1111/jcmm.70807

Figure Lengend Snippet: Prediction of potential effective small‐molecule drugs and drug sensitivity. (A, B) A total of 8 CTRP‐derived and 20 PRISM‐derived compounds were obtained from drug resistance prediction models based on the CTRP2.0 (A) and PRISM (B) datasets. (C) The molecular docking model illustrates the interaction between the PLK1 protein and the small molecule drug SB‐743921. (D) The molecular docking model demonstrates the interaction between the PRC1 protein and the small molecule drug ispinesib. (E) The drug sensitivity of three frequently utilised clinical drugs, namely cisplatin, paclitaxel and sorafenib, was assessed. (F) The IC50 values for commonly used drugs of chemotherapy and targeted therapy were compared between the two risk groups.

Article Snippet: Subsequently, antigen retrieval was carried out on slides immersed in the sodium citrate buffer (10 mM sodium citrate, 0.05% Tween 20, pH 6.0) under high pressure for 2 min. After cooling to room temperature (RT), slides were washed with TBST/0.5% Tween and incubated with 3% H 2 O 2 for 15 min, and then blocked with 1% (w/v) BSA Fraction V (ST023, Beyotime) and 10% goat serum (v/v) (B900780, Proteintech) in PBS for 1 h. Slides were incubated with the following primary antibodies against HMMR (Proteintech, 1:200), PLK1 (Proteintech, 1:200) and PRC1 (Proteintech, 1:200) overnight at 4°C, followed by incubation with the secondary antibodies: Alexa Fluor 488‐conjugated affiniPure Goat antiRabbit IgG (H + L) (1:200, 115‐585‐146, Jackson ImmunoResearch) for 1 h, and nuclei‐staining with DAPI (1:200, D9542, Sigma) at RT for 10 min.

Techniques: Derivative Assay

Journal: Journal of Cellular and Molecular Medicine

Article Title: Investigating Liquid–Liquid Phase Separation in Lung Adenocarcinoma to Improve Prognostic Accuracy and Treatment Efficacy

doi: 10.1111/jcmm.70807

Figure Lengend Snippet: Experimental validation of the key genes in the LLPSAS. (A) The intrinsic disorder regions (IDRs) of the proteins HMMR, PLK1 and PRC1 were visualised using the PSIPRED website. (B) IHC staining of HMMR, PLK1 and PRC1 in tumour sections and para‐tumour sections; images were captured at 20×. (C) Immunofluorescence staining of HMMR, PLK1 and PRC1 was performed in SPC‐A‐1 cells. (D) Immunofluorescence staining of HMMR, PLK1 and PRC1 in tumour sections and para‐tumour sections; representative images of the existence of fluorescent foci on these genes were showed in inset, respectively.

Article Snippet: Subsequently, antigen retrieval was carried out on slides immersed in the sodium citrate buffer (10 mM sodium citrate, 0.05% Tween 20, pH 6.0) under high pressure for 2 min. After cooling to room temperature (RT), slides were washed with TBST/0.5% Tween and incubated with 3% H 2 O 2 for 15 min, and then blocked with 1% (w/v) BSA Fraction V (ST023, Beyotime) and 10% goat serum (v/v) (B900780, Proteintech) in PBS for 1 h. Slides were incubated with the following primary antibodies against HMMR (Proteintech, 1:200), PLK1 (Proteintech, 1:200) and PRC1 (Proteintech, 1:200) overnight at 4°C, followed by incubation with the secondary antibodies: Alexa Fluor 488‐conjugated affiniPure Goat antiRabbit IgG (H + L) (1:200, 115‐585‐146, Jackson ImmunoResearch) for 1 h, and nuclei‐staining with DAPI (1:200, D9542, Sigma) at RT for 10 min.

Techniques: Biomarker Discovery, Immunohistochemistry, Immunofluorescence, Staining